Measurement

Part:BBa_K2224003:Design

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-23)


SacB promoter+RBS（BBa_J61100）+GFP +Terminator（BBa_B0015）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1204


Design Notes

Since there are past experiments show that SacB promoter has a weak ability in E. coli, we wanted to test 'how weak' this promoter is in the expression system.


Source

Bacillus subtilis subsp. subtilis str. 168.

References